The 61-year-old won the honors for "Best Adapted Screenplay" for his work on 2018's BlackKklansman.
Lee shares the award with co-writers Charlie Wachtel, David Rabinowitz and Kevin Wilmott.
Jackson got to deliver plenty of good news to friend and longtime Knicks fan Spike Lee on Sunday night.
"From 1619 to 2019, 400 years, 400 years that our ancestors were stolen from mother Africa and brought to Jamestown, Virginia enslaved", Lee said.
The BlacKkKlansman director was reported to have stood up and waved his hands in disgust during Sunday's Oscars ceremony, when Julia Roberts announced Green Book as the victor of the night's top prize.
"Before the world tonight I give praise to our ancestors, who helped build this country".
Reds to face tough test against Manchester United
Smalling spurning the best of them by failing to get on the end of a Lukaku cross in the final minute of normal time. Manchester United put on a spirited display to earn a 0-0 draw with Liverpool today at Old Trafford.
Back on the Academy stage, Lee closed out his speech with a simple call to action, reminding audience members about the impending presidential elections and ending with a small shoutout for his first hit film. "Make the moral choice between love versus hate", he said.
The legendary filmmaker had previously addressed Green Book as he spoke about Do The Right Thing not being nominated for Best Picture in 1990, while Driving Miss Daisy won.
After Green Book topped Lee's own BlackKklansman and several other deserving winners to claim Best Picture, the veteran director looked "visibly angry" and attempted to leave for the exit, according to The Associated Press. In 2016, Lee received an honorary Oscar for his career.
BlacKkKlansman is a searing yet sometimes hilarious broadside against racism with the stranger-than-fiction true story of an African-American police officer who managed to infiltrate the highest levels of the Ku Klux Klan. In the film, John David Washington plays Stallworth, while Adam Driver plays Zimmerman.
Get push notifications with news, features and more.