They earlier had begun dismantling their encampment.
A group called Wet'suwet'en Strong: Hamilton in Solidarity, claimed responsibility for the blockade.
Separately, Crown-Indigenous Relations Minister Carolyn Bennett said the Wet'suwet'en hereditary chiefs have been meeting with one another in B.C. over the last two days after some of them travelled to Ontario and Quebec to meet Mohawk supporters last week.
"I've been meaning to come, but with what he said yesterday, I just said, OK I'm going", said Sarah Dear, who brought small gifts from Mexico she said were symbolic for Indigenous communities there. "The onus is on them".
Other blockades across Canada have led to a near-total shut down of the Canadian rail system, with VIA Rail, Canada's largest passenger railway company, laying off over a thousand workers as a result of the blockades, and shortages have been reported in some areas due to lack of rail traffic.
There has always and continues to be, a willingness from the Tyendinaga Mohawks to discuss an exit strategy of the CN Rail Main Line. The crisis is daily stranding goods worth an an estimated 425 million Canadian dollars ($340 million), according to the Canadian Manufacturers and Exporters trade group.
In a news conference Friday afternoon, Trudeau spoke at length about the need for the barricades to be removed, but he offered no specifics about steps the government would take to make that happen. He said removing the barricades must be done peacefully. He said the prime minister's plea for patience last week prevented police from acting sooner. He said it would be lamentable if there was violence when the barricades are taken down, but added that Canadians can not continue to suffer as a result of the rail shut down.
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The protests have piled pressure on Trudeau, who came to power promising reconciliation with Canada's First Nations, but has supported the country's fossil fuels industry.
"It doesn't mean that the dialogue is closed, far from that, we remain open for dialogue", he said.
Gitxsan Chief Spookwx, who also goes by Norm Stephens, accused the RCMP of "dragging their feet" on demands from the Wet'suwet'en hereditary chiefs.
Police secured the area near Belleville, Ontario, Canadian National Railway Co (CN) said, and one train was allowed to pass in the evening.
After that meeting, Woos says he will signal through a press release and a call to protesters in Tyendinaga whether the hereditary chiefs are satisfied with the measures taken by RCMP to get out of the territory. "There is a difference between inconvenience and injustice - total difference".
Norton has said the injunction will not be executed in Kahnawake, and that the Mohawk Peacekeepers are the only policing agency with jurisdiction in the territory.
On Sunday, the OPP met with the land defenders and told them that if they were still on the blockade at midnight, they would be subject to arrest.