The 18-hole event at Medalist Golf Club in Hobe Sound, Florida, had a delayed start due to rain.
The former pro golfer tweeted that she very almost offered to get an "Only Fans" account (a very X-rated site) if Brady made a par.
Brady, who has a handicap of 8, hit some truly fantastic shots during his team's defeat to Woods and Manning, but there were a few moments he'd probably rather not see again.
Four-time Major victor Brooks Koepka hailed Brady as "the goat" following his birdie from the fairway. "What a fun day for an even better cause!"
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"I would have loved to have had this tournament in a place where they don't like Tom very much, Indianapolis, Denver, Boston after he just betrayed them and broke their hearts", the retired Denver Broncos pro said, taking a jab at Brady's recent decision to leave the New England Patriots.
The goal was to raise $10 million ($A15.3m) for COVID-19 relief funds and online donations sent money climbing with a final figure of $20m ($A30.6m).
"It's something I'll always remember".
It's unclear how both Woods and Mickelson would fare on the football field. Whatever the case was, Brady's finished off a par-5 with an impressive shot that left him with a birdie. He ripped his trousers during the match and even had a few balls fly out of the fairway in a few holes, to the delight of TNT anchor and National Basketball Association vet Charles Barkley who was on the call.